Ingredient g/L
Peptone 5.00
Yeast extract 1.00
Ferric citrate 0.10
Sodium chloride 19.45
Magnesium chloride 8.80
Sodium sulphate 3.24
Calcium chloride 1.80
Potassium chloride 0.55
Sodium bicarbonate 0.16
Potassium bromide 0.08
Strontium chloride 0.034
Boric acid 0.022
Sodium silicate 0.004
Sodium fluorate 0.0024
Ammonium nitrate 0.0016
Disodium phosphate 0.008
Agar 15.00
Final pH (at 25°C) : 7.6 + 0.2
Direction
Suspend 55.25 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and Pour in sterile Petri plates.
Principle
This medium consists not only of minerals as sea water but also has peptone and yeast extract as the better sources of nutrients for the marine bacteria. Agar is the solidifying agent. Marine bacteria are of prime importance in food industry and marine life conservation and hence activity of these bacteria can be studied by the use of this medium.
QC Tests
(I) Dehydrated Medium
Colour : Cream to yellow
Appearance : Homogeneous Free Flowing powder
(II) Rehydrated medium
pH (post autoclaving/heating) : 7.6 ± 0.2
Colour (post autoclaving/heating) : Yellow
Clarity (post autoclaving/heating) : Opalescent
(III) Q.C. Test Microbiological
Cultural characteristics observed after an incubation at 20-25°C for 40-72 hours .
MICROORGANISM (ATCC ) GROWTH
Vibrio fischeri (7744) Good –luxuriant
Vibrio harveyi (14126) Good –luxuriant
Use
For cultivation, isolation and enumeration of heterotrophic marine bacteria.
Storage
Dehydrated medium- below 30°C Prepared medium– Between 2 to 8°C.
| CAS No. | |
|---|---|
| Packing | 500 G |
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ZOBELL MARINE AGAR