- Accurate and easy-to-use
- Quantitate or screen samples
- High specificity
Materials Provided
- 48 antibody-coated microwells (24 per pouch)
- 48 red-marked transfer wells (24 per pouch)
- 5 yellow-labeled bottles of 0, 2.5, 5, 10 and 25 ppm nonfat dry milk (NFDM) controls
- 4 blue-labeled bottles of enzyme-labeled antibody conjugate
- 1 green-labeled bottle of K-Blue® Substrate
- 1 red-labeled bottle of Red Stop solution
- 5 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solvent
- 2 wide-mouth bottles of 40 mL PBS-Tween wash buffer concentrate; each bottle contains enough concentrate to prepare 1 L of wash buffer
- 50 g of extraction additive in a specimen cup
- Plastic scoop to measure extraction additive
Materials Required But Not Provided
- Allergen Extraction Kit (Neogen item 8429)
- 20 disposable plastic extraction bottles
- 20 disposable transfer pipettes
- Shaker water bath adjusted to 60°C ± 1° (140°F) with clamps to hold extraction bottles
- Adjustable 50-200 µL pipettor (Neogen item 9276)
- Pipette tips (Neogen item 9410)
- 12-channel pipettor (Neogen item 9273)
- Timer (Neogen item 9426)
- Microwell strip reader with a 650 nm filter (Neogen item 9303)
- 1 L bottle to prepare washing solution (Neogen item 9472)
- 1 L heat safe bottle to prepare extract solution (Neogen item 9472)
- Paper towels or equivalent absorbent material
- Microwell holder (Neogen item 9402)
- Waterproof marker
- Wash bottle (Neogen item 9400)
- Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
- Scale capable of weighing 5 g ± 0.1 g (Neogen item 9427)
- 3 reagent multichannel pipettor boats (Neogen item 9435)
- Distilled or deionized water