• Accurate and easy-to-use
  • Cost effective microwell format
  • High specificity

Materials Provided

  1. 48 antibody-coated microwells
  2. 48 red-marked transfer wells
  3. 5 yellow-labeled bottles of 0, 5, 10, 20, and 50 ppm gliadin (0, 10, 20, 40 and 100 ppm gluten) controls (0, 12.5, 25, 50, and 125 ng/mL gliadin)
  4. 2 blue-labeled bottles of enzyme-labeled antibody conjugate
  5. 1 green-labeled bottle of K-Blue® Substrate
  6. 1 red-labeled bottle of Red Stop solution
  7. 40 mL of 10 mM PBS-Tween washing reagent in a wide mouth bottle. Each bottle can prepare 1 L in distilled or deionized water (pH 7.4)
  8. 1 foil pouch of 10 mM PBS dry powder containing enough powder to prepare 1 L of dilution solvent
  9. 50 g of extraction additive in 2 specimen cups
  10. Plastic scoop to measure extraction additive

Materials Required But Not Provided

  1. Orbital rotator or shaker to hold 50 cc centrifuge tubes for a 1 g sample, or shaker water bath with clamps adjusted to hold 125 mL (4 oz) extraction bottles for a 2 g sample
  2. Oven or water bath adjustable to 50°C if analyzing heat-processed samples
  3. Scale capable of weighing 1 ± 0.1 g (Neogen item 9427), or scale capable of weighing 0.25 ± 0.01 g if using gliadin cocktail solution (Neogen item 9395)
  4. Pipettor, 50–200 μL adjustable (Neogen item 9276)
  5. Pipettor, 12-channel (Neogen item 9273)
  6. Pipette tips (Neogen item 9410, 9417, 9407)
  7. Three 1 L bottles to prepare washing solution, extract solution, and sample extract dilution solution (Neogen item 9472)
  8. Test tubes to perform sample extract dilution
  9. Timer (Neogen item 8426, 9452)
  10. Microwell reader with a 650 nm filter (Neogen item 9303)
  11. Three reagent boats for 12-channel pipettor (Neogen item 9435)
  12. Microwell strip holder (Neogen item 9402)
  13. Wash bottle (Neogen item 9400)
  14. Paper towels or equivalent absorbent material
  15. Waterproof marker
  16. Distilled or deionized water
  17. Laboratory grade ethanol (190 proof)
  18. Special extraction additive for dark chocolate, cocoa and tannin (Neogen item 8482)

If testing heat treated products:

  1. Gliadin renaturing cocktail solution for heat processed samples (Neogen item 8483, 8483B, 8483S)
Specifications
Brand Veratox®
Analyte Gliadin/Gluten
Platform ELISA
Result Type Quantitative
Limit of Detection 5 ppm gliadin
Testing Time 30.00
Prop 65 Warning
Warning

WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 48 tests
Package Weight 0.80 lb

Documentation

SKUProductTypeLanguageFile
8480Veratox® for GliadinMaterial Safety Data SheetEnglishDownload
8480Veratox® for GliadinUser ManualEnglishDownload

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Veratox® for Gliadin
Veratox® for Gliadin