- Accurate and easy-to-use
- Cost effective microwell format
- High specificity
Materials Provided
- 48 antibody-coated microwells
- 48 red-marked transfer wells
- 5 yellow-labeled bottles of 0, 5, 10, 20, and 50 ppm gliadin (0, 10, 20, 40 and 100 ppm gluten) controls (0, 12.5, 25, 50, and 125 ng/mL gliadin)
- 2 blue-labeled bottles of enzyme-labeled antibody conjugate
- 1 green-labeled bottle of K-Blue® Substrate
- 1 red-labeled bottle of Red Stop solution
- 40 mL of 10 mM PBS-Tween washing reagent in a wide mouth bottle. Each bottle can prepare 1 L in distilled or deionized water (pH 7.4)
- 1 foil pouch of 10 mM PBS dry powder containing enough powder to prepare 1 L of dilution solvent
- 50 g of extraction additive in 2 specimen cups
- Plastic scoop to measure extraction additive
Materials Required But Not Provided
- Orbital rotator or shaker to hold 50 cc centrifuge tubes for a 1 g sample, or shaker water bath with clamps adjusted to hold 125 mL (4 oz) extraction bottles for a 2 g sample
- Oven or water bath adjustable to 50°C if analyzing heat-processed samples
- Scale capable of weighing 1 ± 0.1 g (Neogen item 9427), or scale capable of weighing 0.25 ± 0.01 g if using gliadin cocktail solution (Neogen item 9395)
- Pipettor, 50–200 μL adjustable (Neogen item 9276)
- Pipettor, 12-channel (Neogen item 9273)
- Pipette tips (Neogen item 9410, 9417, 9407)
- Three 1 L bottles to prepare washing solution, extract solution, and sample extract dilution solution (Neogen item 9472)
- Test tubes to perform sample extract dilution
- Timer (Neogen item 8426, 9452)
- Microwell reader with a 650 nm filter (Neogen item 9303)
- Three reagent boats for 12-channel pipettor (Neogen item 9435)
- Microwell strip holder (Neogen item 9402)
- Wash bottle (Neogen item 9400)
- Paper towels or equivalent absorbent material
- Waterproof marker
- Distilled or deionized water
- Laboratory grade ethanol (190 proof)
- Special extraction additive for dark chocolate, cocoa and tannin (Neogen item 8482)
If testing heat treated products:
- Gliadin renaturing cocktail solution for heat processed samples (Neogen item 8483, 8483B, 8483S)