- Accurate and easy-to-use
- Quantitate or screen samples
- High specificity
Materials Provided
- 48 antibody-coated microwells
- 48 red-marked transfer wells
- 6 yellow-labeled bottles of 0, 1, 2.5, 5, 10, and 25 ppm coconut controls
- 2 blue-labeled bottles of enzyme-labeled antibody conjugate
- 1 green-labeled bottle of K-Blue® Substrate
- 1 red-labeled bottle of Red Stop solution
- 5 foil pouches of phosphate-buffered saline (PBS) powder. Each pouch contains PBS powder sufficient to prepare 1.0 L of 10 mM PBS buffer at pH 7.4.
- 40 mL of 10 mM PBS-Tween washing reagent concentrate in a wide mouth bottle. Each bottle contains wash reagent concentrate sufficient to prepare 1 L in distilled or deionized water (pH 7.4)
- 50 g of extraction additive in a specimen cup
- Plastic scoop to measure extraction additive
Materials Required But Not Provided
- Allergen Extraction Kit (Neogen item 8429)
- 20 disposable plastic extraction bottles
- 20 sample collection tubes (12 x 75 mm) with caps
- Shaker water bath capable of maintaining 60 ± 1°C with holders for 250 mL disposable plastic bottles (Neogen items 9298, 9299)
- Whatman No. 4 filters or equivalent (Neogen item 9429)
- Centrifuge (optional)
- Pipettor, 50–200 µL, adjustable (Neogen item 9276)
- Pipettor, 12-channel (Neogen item 9273)
- Pipette tips (Neogen item 9410, 9407, 9417)
- Timer (Neogen items 9426, 9452)
- Microwell reader with a 650 nm filter (Neogen item 9303)
- 1 L bottle to prepare washing solution (Neogen item 9472)
- 1 L heat safe bottle to prepare extract solution (Neogen item 9472)
- Paper towels or equivalent absorbent material
- Microwell holder (Neogen item 9402)
- Waterproof marker
- Wash bottle (Neogen item 9400)
- Distilled or deionized water
- 3 reagent boats for 12-channel pipettor (Neogen item 9435)
- Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
- Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)