Ingredients : gms/lit.
Pancreatic digest of casein 17.00
Papaic digest of soyabean meal 3.00
Sodium chloride 5.00
Dipotassium phosphate 2.50
Dextrose 2.50
Final pH (at 25°C) : 7.3 + 0.2
Directions :
Suspend 30 gms. in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle :
The combination of pancreatic digest of casein and papaic digest of soyabean meal makes the medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Dextrose and dibasic potassium phosphate serve as the carbohydrate source and the buffer, respectively in the medium. Sodium chloride maintains the osmotic balance of the medium.