Ingredients: gms/lit.
Yeast extract
Dextrose
Agar
5.00
20.00
12.00
Final pH (at 25°C) : 7.0 + 0.2
Directions :
Suspend 18.5 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely.
Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and
aseptically add reconstituted contents of one vial of Oxytetra Selective Supplement. Mix
well and pour into sterile Petri plates.
Principles :
Yeast extract provides essential growth nutrients. Dextrose acts as carbon and energy source. Low
pH helps to reduce the bacterial flora. Oxytetracycline makes the medium more selective by
inhibiting the growth of Lactobacilli encountered in milk and milk-products at low pH.
| Packing | 500 G |
|---|
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Reagents
ß-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
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Reagents
Calgon S; Glassy sodium; Graham's salt; Hexasodium metaphosphate; Metaphosphoric acid hexasodium salt
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