Ingredients: gms/lit.
Peptone (meat or casein) 1.00
Sodium chloride 4.30
Disodium hydrogen phosphate dihydrate 7.23
Potassium dihydrogen phosphate 3.60
Final pH (at 25°C) : 7.0 + 0.2

Directions :
Suspend 14.64 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified /distilled water. Heat if necessary, to dissolve the medium completely. For preparation of nonfatty products insoluble in water, add 0.1 % w/v Polysorbate 80 to assist the suspension of poorly wettable substances. Dispense in tubes or flasks or as desired and sterilize by autoclaving at 15 lbs pressure 121°C for 15 minutes or as per validated cycle.

Principle :
Buffered Peptone Water is a pre-enrichment medium designed to help recovery of sub-lethally damaged Salmonellae before transfer to a selective medium. This pre-enrichment medium is free from inhibitors and is well buffered and provides conditions for resuscitation of the cells that have been injured by processes of food preservation. Pre-enrichment in Buffered Peptone Water at 37°C for 18 hours before selection in Tetrathionate Brilliant Green Bile Broth showed superior results compared with direct selection method. The composition of Buffered Peptone Water with NaCl medium is as per IP and EP specifications recommended to dilute the sample for microbial examination. Depending on the amount of fat in the sample to examine the kind and quantity of emulsifying agent to be used. These pre-enrichment media contain peptone as a source of carbon, nitrogen, vitamins and minerals. Sodium chloride maintains the osmotic balance and phosphates buffer the medium. The broth is rich in nutrients and produces high resuscitation rates for sub-lethally injured bacteria and supports intense growth. The phosphate buffer system prevents bacterial damage due to changes in the pH of the medium.

Packing

500 G

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Platforms

Platform PRSC-10

10 tubes 25-30 mm diameter (50 ml tubes).

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Centrifuge Adapters

Adapter set (2 pcs) – AP-96

2 adapters for 96-well semi-skirted and non-skirted PCR plates - made of Ertacetal® C. Autoclavable.

Bottle Top Dispensers

CAPPForte Bottle Top Dispenser

CAPPForte bottle top dispenser is the ideal choice for handling strong and corrosive chemicals. Made of highly chemical resistant materials, such as PTFE, PFA, Borosilicate and Platinum, CAPPForte bottle top dispenser can be used for processing acids, solvents and bases on standard and sterile applications, as it is also autoclavable at 121° C / 250° F.

This product has multiple variants. The options may be chosen on the product page

Mechanical Pipettes

CAPPBravo Pipette

CAPPBravo automatic single-channel pipette combines lightweight ergonomic design with the latest innovations in single channel pipettes, making it one of the most user friendly automatic pipettes on the market today. CAPPBravo is both available as variable volume pipette (9 volume ranges starting from 0.1µl up to 10mL), as well as a fixed volume pipette. Constructed of the highest quality autoclavable materials, this mechanical pipette features feather light plunger and ejection forces, as well as smooth tip mounting. Ergonomically constructed hook ensures comfortable handling. The built-in oversized indicator makes volume monitoring and precise adjustment easy. CAPPBravo manual pipette offers ultra micro volumes with metal shafts and double o-rings to increase the durability of the pipettes. Experience CAPP’s symphony of precise measurements and see for yourself why it is called Bravo!

This product has multiple variants. The options may be chosen on the product page

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BUFFERED SODIUM CHLORIDE PEPTONE SOLUTION

SKU: DM0990500 Category: