Ingredients Gms/Ltr :
Lactose 15.00
Agar 13.00
Peptone 7.00
Sodium chloride 5.00
Phenol red 0.04
Brilliant green 0.005
* Dehydrated powder, hygroscopic in nature, store in a dry place, in tightly-sealed containers below 25°C
and protect from direct Sunlight.
Instructions for Use :
Dissolve 40.00gms in 1000 ml distilled water. Gently heat to boiling with gentle swirling and dissolve the
medium completely. Sterilize by autoclaving at 15 psi (121°C) for 10 minutes. Cool to 45 - 50°C and pour
into sterile Petri plates.
Appearance: Red - brown colour, clear gel
pH at 25°C: 6.5 ± 0.2
Principle
BPL AGAR (BRILLIANT GREEN PHENOL RED LACTOSE AGAR) is used for isolation and
identification of Salmonellae except Salmonella typhi in faeces, urine and milk and foodstuffs by
separating lactose and sucrose negative bacteria. The Peptone provides the essential nutrient nitrogen for
growth of microorganisms. Brilliant green in the medium inhibits accompanying microorganisms.
Medium contains Lactose, whose degradation to acid is indicated by the pH indicator Phenol red, which
changes its colour to yellow. The indicator exhibits a deep red colour in the alkaline range. Agar is a
solidifying agent. Sodium chloride maintains the osmotic balance of the medium. The growth of the
accompanying Gram-positive microbial flora, Salmonella typhi and Shigella is largely inhibited by brilliant
green. ADAM (1966) recommended that 0.2 % sodium deoxychlolate should be added to the culture
medium to inhibit the swarming of Proteus colonies.
| CAS No. | |
|---|---|
| HS Code | 38210000 |
| Packing | 500 G |
Recently Viewed
(1-Hexadecyl)trimethylammonium bromide; Trimethylhexacecylammonium bromide; Cetrimonium Bromide; CTAB
Ceric ammonium sulphate, Cerium(IV) ammonium sulphate
Reagents
Ammonium heptamolybdate tetrahydrate, Molybdic acid ammonium salt tetrahydrate
Reagents
Red No. 3, Solvent Red 140, Acid Red 51, FD&C #3 C.I. Food Red 14; 3',6'-Dihydroxy-2',4',5',7'-tetraiodospiro[isobenzofuran-1(3H0,9'-[9H]xanthen]-3-one disodium salt
Products Best Selling
For the separation and purification of samples of cells, macromolecular proteins, blood, body fluids, DNA/RNA, plasmids, bacteria and viruses.
Sterile TPP TubeSpin® Bioreactor vessel, 50 ml, with pH and O2 sensor spots, 1 pce.
The ES-20 is a compact bench–top Shaker–Incubator used for mixing of biological liquids as well as for incubation and cultivation of biological liquids according to the operator set program
Applicable for storing wholeblood, medicines, biological products and other laboratory products that need to be stored at 4℃. Suitable for the storage of blood and blood articles in areas that have power shortages.
LN2 backup cooling system is an independent refrigeration system for a ULT freezer. When there is a loss of power or the temperature of the freezer rises to the high alarm set point, the LN2 backup system can be automatically activated to inject LN2 into the chamber to maintain the freezer temperature. The backup system operates on a rechargeable battery when there is a loss of main power.
Products Favourite
The ES-20 is a compact bench–top Shaker–Incubator used for mixing of biological liquids as well as for incubation and cultivation of biological liquids according to the operator set program
For the separation and purification of samples of cells, macromolecular proteins, blood, body fluids, DNA/RNA, plasmids, bacteria and viruses.
Sterile TPP TubeSpin® Bioreactor vessel, 50 ml, with pH and O2 sensor spots, 1 pce.
Applicable for blood stations, hospitals, CDC's, scientific research institutes, chemical industry and other related industries. The -30°C Biomedical Freezer can cryopreserve plasma, biological products, components, materials, and other items that need to be kept at low temperature.
This product has multiple variants. The options may be chosen on the product page
LN2 backup cooling system is an independent refrigeration system for a ULT freezer. When there is a loss of power or the temperature of the freezer rises to the high alarm set point, the LN2 backup system can be automatically activated to inject LN2 into the chamber to maintain the freezer temperature. The backup system operates on a rechargeable battery when there is a loss of main power.
Brillant Green Penol Red Lactose Agar – BPL Agar