Materials Provided
- Walnut Standard S1 2.4 ppm Walnut
- Walnut Standard S2 6 ppm Walnut
- Walnut Standard S3 12 ppm Walnut
- Walnut Standard S4 60 ppm Walnut
- Walnut Standard S5 120 ppm Walnut
- Walnut Biotin
- Avidin Peroxidase Conjugate
- TMB Substrate
- Wash Solution Concentrate
- Diluent Concentrate Type 8
- Stop Solution
- Walnut plate 48 Microwells (6 x 8 strips + frame)
- Package insert
- Blank result form
Materials Required But Not Provided
- Sodium chloride
- Tris (hydroxymethyl) methylamine
- 1M hydrochloric acid
- Teleost gelatin (Sigma G7765)
- Pestle and mortar, stomacher or suitable grinder/mill, WHICH CAN BE EASILY CLEANED after use to eliminate the possibility of cross-contamination between samples
- Miscellaneous laboratory plastic and/or glassware, including measuring cylinders, pipettes, disposable Pasteur pipettes, plate seals and containers suitable for food extracts
- pH Meter
- Water bath capable of maintaining 60°C ± 2°C 3
- Wrist action or similar shaker
- Centrifuge and appropriate centrifuge/microfuge tubes for clarifying sample extract
- Precision micropipette(s) capable of delivering 50 and 100 microliters, plus disposable tips
- 96 well plate seals
- Microwell washer (e.g. NUNC Immuno Wash 8) or wash bottle
- Microwell plate reader, fitted with 450 nm interference filter (Calibrate regularly. Fixed error <± 0.03; variable error><± 1%)>