Materials Provided
- Peanut Standard S1 1 ppm peanut
- Peanut Standard S2 2 ppm peanut
- Peanut Standard S3 5 ppm peanut
- Peanut Standard S4 10 ppm peanut
- Peanut Standard S5 20 ppm peanut
- Peanut Control
- Biscuit crumb
- Peanut Biotin
- Avidin Peroxidase Conjugate
- TMB Substrate
- Wash Solution Concentrate
- Diluent Concentrate Type 8
- Stop Solution
- Peanut plate 96 microwells (12 x 8 strips + frame)
- Package insert
- Blank result form
Materials Required But Not Provided
- Sodium chloride (Neogen® item 7725G)
- Tris (hydroxymethyl) methylamine (Sigma 93349)
- 1M hydrochloric acid (Fisher 393260)
- Teleost gelatin (Sigma G7765)
- Pestle and mortar, Stomacher or suitable grinder/mill that can be easily cleaned after use to eliminate the possibility of cross-contamination between samples
- Miscellaneous laboratory plastic and/or glassware, including measuring cylinders, pipettes, disposable Pasteur pipettes, plate seals and containers suitable for food extracts
- pH Meter
- Water bath capable of maintaining 60 ± 2°C 3
- Wrist action or similar shaker
- Centrifuge and appropriate centrifuge/microfuge tubes for clarifying sample extract
- Micropipette(s), 50–100 µL (Neogen item 9276)
- Disposable tips for micropippettes, 50–100 µL (Neogen items 9407, 9410, 9417)
- Microwell washer (e.g., NUNC Immuno Wash 8) or wash bottle
- Microwell plate reader, fitted with 450 nm interference filter (calibrate regularly)