Materials Provided
- Milk Protein Standard
- BLG Control
- BLG Biotin
- Avidin Peroxidase Conjugate
- TMB Substrate
- Wash Solution Concentrate
- Diluent Concentrate Type 10
- Stop Solution
- BLG Plate
- Package Insert
- Worksheet
Materials Required But Not Provided
- 0.05M carbonate / bicarbonate buffer pH 9.6 OR equivalent buffer tablets e.g. Sigma C3041
- Purified water
- Fish gelatin (Sigma G7765) optional
- Ultra Turrax or similar homogenizer, and/or Waring or similar blender or food processor, and/or stomacher or similar homogenizer
- Miscellaneous laboratory plastic and/or glassware, including measuring cylinders, pipettes, disposable Pasteur pipettes, plate seals and containers suitable for food extracts
- pH meter
- Water bath capable of maintaining 60°C ± 2°
- Wrist action or similar shaker
- Validated microwell plate shaker set at 700 – 800 rpm
- Centrifuge and appropriate centrifuge/microfuge tubes for clarifying sample extract
- Precision micropipette(s) capable of delivering 50 and 100 microlitres, plus disposable tips
- Microwell washer (e.g. NUNC Immuno Wash 8) or wash bottle
- Microwell plate reader, fitted with 450 nm interference filter (Calibrate regularly. Fixed error <± 0.03; variable error><± 1%)>