Materials Provided

  1. Milk Protein Standard
  2. BLG Control
  3. BLG Biotin
  4. Avidin Peroxidase Conjugate
  5. TMB Substrate
  6. Wash Solution Concentrate
  7. Diluent Concentrate Type 10
  8. Stop Solution
  9. BLG Plate
  10. Package Insert
  11. Worksheet

Materials Required But Not Provided

  1. 0.05M carbonate / bicarbonate buffer pH 9.6 OR equivalent buffer tablets e.g. Sigma C3041
  2. Purified water
  3. Fish gelatin (Sigma G7765) optional
  4. Ultra Turrax or similar homogenizer, and/or Waring or similar blender or food processor, and/or stomacher or similar homogenizer
  5. Miscellaneous laboratory plastic and/or glassware, including measuring cylinders, pipettes, disposable Pasteur pipettes, plate seals and containers suitable for food extracts
  6. pH meter
  7. Water bath capable of maintaining 60°C ± 2°
  8. Wrist action or similar shaker
  9. Validated microwell plate shaker set at 700 – 800 rpm
  10. Centrifuge and appropriate centrifuge/microfuge tubes for clarifying sample extract
  11. Precision micropipette(s) capable of delivering 50 and 100 microlitres, plus disposable tips
  12. Microwell washer (e.g. NUNC Immuno Wash 8) or wash bottle
  13. Microwell plate reader, fitted with 450 nm interference filter (Calibrate regularly. Fixed error <± 0.03; variable error><± 1%)>
Specifications
Brand BioKits
Analyte BLG
Platform ELISA
Result Type Quantitative
Limit of Detection <2 ppm BLG
Testing Time 120.00
Prop 65 Warning
Warning

WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 36 tests
Package Weight 1.00 lb

Documentation

SKUProductTypeLanguageFile
902061YBioKits BLG Assay KitMaterial Safety Data SheetEnglishDownload
902061YBioKits BLG Assay KitUser ManualEnglishDownload

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