• Minimal equipment required
  • Screen single or multiple samples at one time
  • Test food, rinses or swabs

Materials Provided

  1. 24 antibody-coated microwells
  2. 2 yellow-labeled bottles of controls made from nonfat dried milk; 1 bottle at a concentration of 5 ppm and 1 bottle at 10 ppm (see procedural note No. 1)
  3. 1 blue-labeled bottle of enzyme-labeled antibody conjugate
  4. 1 green-labeled dropper bottle of K-Blue® Substrate solution
  5. 1 red-labeled dropper bottle of Red Stop solution
  6. 20 unlabeled empty sample dropper bottles with tips
  7. 3 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solution
  8. 1 wide-mouth bottle of 40 mL PBS-Tween wash buffer concentrate; a bottle contains enough concentrate to prepare 1 L of wash buffer
  9. 50 g of extraction additive in a specimen cup
  10. Plastic scoop to measure extraction additive

Materials Required But Not Provided

  1. Allergen Extraction Kit (Neogen® item 8429)
    1. 20 disposable extraction bottles
    2. 20 disposable transfer pipettes
  2. Allergen Environmental Swabbing Kit (Neogen item 8432S)
    1. 100 sterile swabs
    2. 100 disposable dropper tips
  3. Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)
  4. Shaker water bath, hot plate or equivalent heat source capable of maintaining 60 ± 1°C
  5. Thermometer
  6. Two 1 L bottles to prepare washing and extraction solutions (Neogen item 9472)
  7. Paper towels or equivalent absorbent material
  8. Microwell holder (Neogen item 9402)
  9. Waterproof marker
  10. Wash bottle (Neogen item 9400)
  11. Distilled or deionized water
  12. Timer (Neogen item 9426)
  13. Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
  14. Pipettor, 100 µL (Neogen item 9272, 9278, 9276)
  15. Pipette tips (Neogen item 9410, 9407, 9417)
  16. Vortex (Neogen item 9494)
  17. 50 mL sample tubes (Neogen item 9381)
Specifications
Brand Alert®
Analyte Milk
Platform ELISA
Result Type Qualitative
Limit of Detection 5 ppm, 10 ppm NFDM
Testing Time 30.00
Prop 65 Warning
Warning

WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 20 tests
Package Weight 0.60 lb

Documentation

SKUProductTypeLanguageFile
8471Alert® for Total MilkMaterial Safety Data SheetEnglishDownload
8471Alert® for Total MilkUser InstructionsEnglishDownload
8471Alert® for Total MilkUser ManualEnglishDownload

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Alert® for Total Milk

SKU: 8471 Category: Brand:

Alert® for Total Milk Allergen is used as a screening tool to test raw ingredients, in clean-in-place rinses, on environmental surfaces, or product throughout the production process, for the presence of milk residue.

Neogen

Alert® for Total Milk
Alert® for Total Milk