- Minimal equipment required
- Screen single or multiple samples at one time
- Test food, rinses or swabs
Materials Provided
- 24 antibody-coated microwells
- 2 yellow-labeled bottles of controls made from nonfat dried milk; 1 bottle at a concentration of 5 ppm and 1 bottle at 10 ppm (see procedural note No. 1)
- 1 blue-labeled bottle of enzyme-labeled antibody conjugate
- 1 green-labeled dropper bottle of K-Blue® Substrate solution
- 1 red-labeled dropper bottle of Red Stop solution
- 20 unlabeled empty sample dropper bottles with tips
- 3 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solution
- 1 wide-mouth bottle of 40 mL PBS-Tween wash buffer concentrate; a bottle contains enough concentrate to prepare 1 L of wash buffer
- 50 g of extraction additive in a specimen cup
- Plastic scoop to measure extraction additive
Materials Required But Not Provided
- Allergen Extraction Kit (Neogen® item 8429)
- 20 disposable extraction bottles
- 20 disposable transfer pipettes
- Allergen Environmental Swabbing Kit (Neogen item 8432S)
- 100 sterile swabs
- 100 disposable dropper tips
- Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)
- Shaker water bath, hot plate or equivalent heat source capable of maintaining 60 ± 1°C
- Thermometer
- Two 1 L bottles to prepare washing and extraction solutions (Neogen item 9472)
- Paper towels or equivalent absorbent material
- Microwell holder (Neogen item 9402)
- Waterproof marker
- Wash bottle (Neogen item 9400)
- Distilled or deionized water
- Timer (Neogen item 9426)
- Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
- Pipettor, 100 µL (Neogen item 9272, 9278, 9276)
- Pipette tips (Neogen item 9410, 9407, 9417)
- Vortex (Neogen item 9494)
- 50 mL sample tubes (Neogen item 9381)