Ingredient g/L
Pancreatic digest of casein 17.00
Papaic digest of soyabean meal 3.00
Sodium chloride 5.00
Dipotassium phosphate 2.50
Dextrose 2.50
Final pH (at 25°C) : 7.3 + 0.2
Direction
Suspend 30 gms. in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle
The combination of pancreatic digest of casein and papaic digest of soyabean meal makes the medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Dextrose and dibasic potassium phosphate serve as the carbohydrate source and the buffer, respectively in the medium. Sodium chloride maintains the osmotic balance of the medium.
QC Tests
(I) Dehydrated Medium
Colour : Cream to light yellow
Appearance : Homogeneous Free Flowing powder
(II) Rehydrated medium
pH (post autoclaving/heating) : 7.3 ± 0.2
Colour (post autoclaving/heating) : Light yellow
Clarity (post autoclaving/heating) : Clear
(III) Q.C. Test Microbiological
Cultural characteristics observed after 18 – 48 hours at 35-37°C.
MICROORGANISM (ATCC ) GROWTH
Escherichia coli (25922) Luxuriant
Escherichia coli (8739) Luxuriant
Escherichia coli (NTCC9002) Luxuriant
Salmonella abony (6017 Luxuriant
Salmonella typhimurium (14028) Luxuriant
Staphylococcus aureus (6538) Luxuriant
Pseudomonas aeruginosa (27853) Luxuriant
Pseudomonas aeruginosa (9027) Luxuriant
Candida albicans (10231 ) Luxuriant
Staphylococcus aureus (25923) Luxuriant
Bacillus subtilis (6633) Luxuriant
Staphylococcus aureus (6538) Luxuriant
Micrococcus luteus (9341) Luxuriant
Use
A general purpose medium used for cultivation of a wide variety of microorganisms and sterility testing of molds and lower bacteria.
Storage
Dehydrated medium- below 30°C Prepared medium– Between 2 to 8°C.
| CAS No. | |
|---|---|
| Packing | 500 G |
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SOYABEAN CASEIN DIGEST MEDIUM (TRYPTONE SOYA BROTH)