Ingredient g/L
Peptic digest of animal tissue 1.00
Sodium chloride 8.50
Final pH (at 25°C) : 7.0+ 0.2
Directions
Suspend 9.5 grams in 1000 ml purified/distilled water. Heat if necessary, to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and dispense into tubes or flasks as desired.
Principle
The low concentration of peptic digest of animal tissue helps to maintain the organisms for 1-2 hours of dilution without multiplication. The isotonic property of the diluent ensures the recovery of organisms from various sources, which may be vulnerable in distilled water or aqueous suspensions.
QC Tests
(I) Dehydrated Medium
Colour : White to pale yellow
Appearance : Homogeneous Free Flowing powder
(II) Rehydrated medium
pH (post autoclaving/heating) : 7.0 ± 0.2
Colour (post autoclaving/heating) : Light yellow to colourless
Clarity (post autoclaving/heating) : Clear solution without any precipitate
(III) Q.C. Test Microbiological
Cultural characteristics observed on Soyabean Casein Digest Agar , after an incubation at 35-37°C for 18-24 hours of cultures suspended in Maximum Recovery Diluent for 30 minutes.
MICROORGANISM (ATCC ) RECOVERY (AFTER 30 MINUTES)
Escherichia Coli (25922) no change in numbers
Staphylococcus aureus (25923) no change in numbers
Use
It is a protective and isotonic medium used for maximal recovery of microorganisms from
a variety of sources.
Storage
Dehydrated medium- below 30°C Prepared medium– Between 2 to 8°C
| CAS No. | |
|---|---|
| Packing | 500 G |
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