- Accurate and easy-to-use
- Cost effective microwell format
- High specificity
Materials Provided
- 48 antibody-coated microwells (24 per pouch)
- 48 red-marked transfer wells (24 per pouch)
- 5 yellow-labeled bottles of 0, 2.5, 5, 10, 25 ppm egg controls
- 2 blue-labeled bottles of enzyme-labeled antibody conjugate
- 1 green-labeled bottle of K-Blue® Substrate
- 1 red-labeled bottle of Red Stop solution
- Foil pouches of 10 mM PBS dry powder extraction solvent. Each pouch is enough to prepare 1 L in distilled or deionized water (pH 7.4)
- 40 mL of 10 mM PBS-Tween washing reagent in a wide mouth bottle. Each bottle is enough to prepare 1 L in distilled or deionized water
- Extraction additive in a specimen cup
- Plastic scoop to measure extraction additive
- Directions for use
Materials Required But Not Provided
- Allergen Extraction Kit (Neogen® item 8429)
- 20 disposable plastic extraction bottles
- 20 disposable transfer pipettes
- Allergen Environmental Swabbing Kit (Neogen item 8432S)
- 100 sterile sawbs
- 100 dropper tips
- Shaker water bath capable of maintaining 60 ± 1°C with clamps to hold 250 mL extraction bottles (Neogen item 9298, 9299)
- Whatman no. 4 filters or equivalent (Neogen item 9429)
- Centrifuge (optional)
- Pipettor, 50–200 µL, adjustable (Neogen item 9276)
- Pipettor, 12-channel (Neogen item 9273)
- Pipette tips (Neogen item 9410, 9407, 9417)
- Timer (Neogen item 9426)
- Microwell reader with a 650 nm filter (Neogen item 9303)
- Bottle, 1 L, to prepare washing solution (Neogen item 9472)
- Bottle, 1 L heat safe, to prepare extract solution (Neogen item 9472)
- Paper towels or equivalent absorbent material
- Microwell holder (Neogen item 9402)
- Waterproof marker
- Wash bottle (Neogen item 9400)
- Distilled or deionized water
- Three reagent boats for 12-channel pipettor (Neogen item 9435)
- Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
- Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)