Chemicals

Specifications

COMPOSITION Ingredients Gms / Ltr Peptic digest of animal tissue 10.000 Yeast extract 5.000 Sodium taurocholate 5.000 Sodium thiosulphate 10.000 Sodium chloride 20.000 Sodium lauryl sulphate 0.200 Sodium citrate 10.000 Sucrose 20.000 Bromo thymol blue 0.040 Thymol blue 0.040 Agar 20.000 Instruction for use :  Dissolve 100.28 grams in 1000 ml distilled water.  Heat to boiling to dissolve the medium completely, do not autoclave.  Mix well and pour into sterile Petri plates Principle The medium contains peptic digest of animal tissue and yeast extract, which provide nitrogenous compounds, vitamin B complex and other essential growth nutrients. Sucrose is added as a fermentable sugar. Sodium citrate, sodium lauryl sulphate, sodium taurocholate and sodium thiosulphate as well as high alkalinity of the medium inhibit most of the contaminating organisms. Bromothymol blue and thymol blue are the pH indicators. The alkaline pH of the medium and higher concentration of sodium chloride improves the recovery of Vibrio parahaemolyticus. Sucrose fermenting organisms like V. cholerae and V. alginolyticus produces yellow coloured colonies. Vibrio parahaemolyticus is a sucrose non-fermenting organism and produces blue-green colonies, as does V. vulnificus. Occasionally a few enteric sucrose nonfermenters may exhibit growth e.g. Proteus group.

Specifications

Ingredients gms/lit Soya Peptone 4.50 Sodium chloride 8.00 Potassium dihydrogen phosphate 0.60 Dipotassium phosphate 0.40 Magnesium chloride hexahydrate 29.00 Malachite green 0.036 Final pH (at 25°C) 5.2 + 0.2 Colour Pale greenish yellow

Specifications

Ingredients Gms/Ltr : Agar 20.00 Peptone 10.00 Bismuth sulphite 8.00 Beef extract 5.00 Dextrose 5.00 Disodium phosphate 4.00 Ferrous sulphate 0.30 Brilliant Green 0.025 * Dehydrated powder, hygroscopic in nature, store in a dry place, in tightly-sealed containers below 25°C and protect from direct Sunlight. Instructions for Use : Dissolve 52.3gms in 1000ml of distilled water. Gently heat to boiling with gentle swirling and dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C prior to dispense. Appearance: Greenish yellow colour, solution opalescent with flocculent precipitate pH (at 25°C): 7.7 ± 0.2 Principle : BISMUTH SULPHITE AGAR is used for the selective isolation of Salmonella spp. Salmonella constitute the most taxonomically complex group of bacteria among Enterobacteriaceae. Bismuth Sulphite Agar is a modification of Wilson and Blair formula. Also this medium favors use of larger inoculum as compared to other selective media, as it has unique inhibitory action towards gram-positive organisms and coliforms. Peptone and Beef extract serve as sources as carbon, nitrogen, vitamins and essential growth factors. Disodium phosphate acts as a buffereing agent. Dextrose is a fermentable carbohydrate source of the growth of microorganisms. Bismuth Sulphite Indicator and Brilliant Green are complementary, inhibiting to Gram-positive bacteria and coliforms, allowing Salmonella spp. to grow. Ferrous Sulphate is used for H2S production. When H2S is present, the iron incorporated in medium is precipitated, and positive cultures produce the characteristic brown to black color with metallic sheen. Agar is the solidifying agent.

Specifications

Calcium carbonate g/L 38.70 Sodium thiosulphate (anhydrous) g/L 30.45 Enzymatic digest of casein g/L 8.60 Ox bile g/L 4.78 Meat Extract g/L 4.30 Sodium chloride g/L 2.60 Brilliant green g/L 0.0096

Specifications

Agar g/L 20.0 Proteose peptone g/L 20.0 Di-potassium hydrogen phosphate g/L 1.50 Magnesium sulfate g/L 1.50

Specifications

Potassium sulfate g/L 10.00 Magnesium chloride, anhydrous g/L 1.64 Proteose peptone g/L 20.00 Agar g/L 15.00 pH at 25°C 7.3 ± 0.2 Colour Cream to yellow Appearance Homogeneous Free Flowingpowder

Specifications

Part I: Acetamide 10.00 g/L Part II: Sodium chloride 5.00 g/L Dipotassium hydrogen phosphate 1.39 g/L Potassium dihydrogen phosphate 0.73 g/L Magnesium sulfate 0.5 g/L Phenol red 0.012 g/L

Specifications

Peptic digest of animal tissue g/L 23.00 Lithium chloride g/L 15.00 Agar g/L 13.00 Mannitol g/L 10.00 Sodium chloride g/L 5.00 Starch g/L 1.00 Esculin g/L 0.80 Dextrose g/L 0.50 Ammonium ferric citrate g/L 0.50 Phenol red g/L 0.08

Specifications

Sodium chloride g/L 20.00 Disodium hydrogen phosphate g/L 9.57 Casein enzymic hydrolysate g/L 5.00 Peptic digest of animal tissue g/L 5.00 Meat extract g/L 5.00 Yeast extract g/L 5.00 Lithium chloride g/L 3.00 Monopotassium phosphate g/L 1.35 Esculin g/L 1.00

Specifications

Agar g/L 15.000 Dextrose g/L 10.000 Peptic digest of animal tissue g/L 5.000 Mono potassium phosphate g/L 1.000 Magnesium sulfate g/L 0.500 Chloramphenicol g/L 0.100 Dichloran g/L 0.002

Specifications

Agar g/L 15.000 Dextrose g/L 10.000 Peptic digest of animal tissue g/L 5.000 Mono potassium phosphate g/L 1.000 Magnesium sulfate g/L 0.500 Chloramphenicol g/L 0.100 Rose bengal g/L 0.025 Dichloran g/L 0.002

Specifications

Ingredients: gms/lit. Yeast extract Dextrose Agar 5.00 20.00 12.00 Final pH (at 25°C) : 7.0 + 0.2 Directions : Suspend 18.5 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add reconstituted contents of one vial of Oxytetra Selective Supplement. Mix well and pour into sterile Petri plates. Principles : Yeast extract provides essential growth nutrients. Dextrose acts as carbon and energy source. Low pH helps to reduce the bacterial flora. Oxytetracycline makes the medium more selective by inhibiting the growth of Lactobacilli encountered in milk and milk-products at low pH.