Dehydrated Media and Bases

Specifications

Peptic digest of animal tissue (Peptone) 6.00 G/L Beef extract 1.500 G/L Yeast extract 3.00 G/L Agar 15.00 G/L Final pH (at 25°C) 7.9 ñ 0.2

Specifications

Peptic digest of animal tissue (Peptone) 5.00 G/L Beef extract 1.500 G/L Yeast extract 1.500 G/L Dextrose 1.000 G/L Sodium chloride 3.500 G/L Dipotassium phosphate 3.680 G/L Potassium dihydrogen phosphate 1.320 G/L Final pH (at 25°C) 7.0 ñ 0.2

Specifications

Peptic digest of animal tissue (peptone) 6.000 G/L Beef extract 1.500 G/L Yeast extract 3.000 G/L Dextrose 1.000 G/L Agar 15.000 G/L pH range 6.40 - 6.80 Reaction reaction of 2.65% w/v aqueous solution at 25°C pH: 6.6 ñ 0.2

Specifications

Peptic digest of animal tissue (Peptone) 6.000 G/L Beef extract 1.500 G/L Yeast extract 3.000 G/L Agar 15.000 Final pH (at 25°C) 6.6 ñ 0.2

Specifications

Peptic digest of animal tissue (Peptone) 10.000 G/L Sodium chloride 10.000 G/L Dextrose 10.000 G/L Beef extract 2.500 G/L Yeast extract 5.000 G/L Agar 25.000 G/L Final pH (at 25°C) 6.1 ñ 0.2

Specifications

Casein enzymic hydrolysate g/L 17.0 Papaic digest of soybean meal g/L 3.0 Sodium chloride g/L 5.0 Dextrose g/L 2.5 Dipotassium phosphate g/L 2.5 Agar g/L 12.0 Final pH (at 25°C) 7.2 +/- 0.2

Specifications

Peptic digest of animal tissue Peptone) 6.000G/L Casein enzymic hydrolysate 4.000G/L Yeast extract 3.000 G/L Beef extract 1.500 G/L Dextrose 1.000 G/L Agar 15.000 G/L Final pH (at 25°C) 6.6 +/- 0.2

Specifications

Proteose peptone 10.000 G/L Casein enzymic hydrolysate 5.000 G/L Yeast exctract 5.000 G/L Dextrose 10.000 G/L Sodium chloride 5.000 G/L Sodium thioglycollate 2.000 G/L Sodium formaldehyde sulfoxylate 1.000 G/L Resazurin 0.002 G/L Agar 15.000 G/L Final pH (at 25°C) 7.2 ñ 0.2 G/L pH range 7.00 - 7.40 Reaction reaction of 3.5% w/v aqueous solution at 25°C pH 7.2 ñ 7.40 Directions : Suspend 53 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. Principle : Proteose peptone, casein enzymichydrolysate, yeast extract provides nitrogen, vitamin and amino acids. Dextrose is a carbohydrate source. This medium contains sodium thioglycollate and sodium formaldehyde sulphoxylate that provide adequate anaerobiosis, which is indicated by resazurin present in the medium. Resazurin imparts pink colour to the medium in presence of oxygen. Brewer devised this medium for use with Brewer anaerobic cover to permit surface growth of anaerobes and microaerophiles on agar without the use of anaerobic jar. For best results, use porous tops on the plates containing the medium during solidification to obtain a dry surface. After inoculation of the medium, cover with Brewer anaerobic petri plate cover. The sealing ring inside the cover should make a perfect contact with the medium and must not be broken before the end of the incubation period.

Specifications

Clarity (2% solution) Clear pH (1% solution) 6.6 +/- 0.5 Moisture NMT 5 % Bacterial Count NMT 5000 Yeast & Molt Count NMT 250 Color light yellow to brownish yellow Appearance Hygroscopic free flowing powder with characteristic odour

Specifications

Solubility (10 mg/mL in water) completely soluble with Clear solution pH (2% solution in water) 6.5 - 7.5 Total Nitrogen (N) Min 12.5 % Loss on drying (at 105°C) Max 5.0 % Appearance Light yellow colored powder

Specifications

pH (1.0 w/v aqueous solution) 6.4 ñ 0.5 Total nitrogen % Min 9.0 à- Amino nitrogen % Min 1.8 Sodium chloride % Max 5.0 Moisture % 5.0 Ash % 14.0

Specifications

pH (2% solution) 6.10 to 7.10 Loss on drying Max 6.0 % Total nitrogen (N) Min 13.5 % Amino nitrogen Min 3.5 % Residue on ignition Max 15.0 % Growth promotion test for pathogens Satisfactory Total viable coun Max 10000 CFU/gram Yeast and mould count Max 100 CFU/gm E.coli. Absent/gm Salmonella species Absent/gm Pseudomonas Absent/gm Appearance Yellow to brown colored powder

Specifications

Total nitrogen (N) Min 11.5 % Amino nitrogen Min 3.2 % Sodium chloride (NaCl) Max 6.0 % pH (2% solution) 6.0 -7.0 Loss on drying (at 105°C) Max 6.0 % Residue on ignition (as SO4) Max 20.0 Salmonella species Absent Aerobic 10000 cfu/gm E.coli Absent Ecoli Absent Appearance Yellow to light brown colored powder

Specifications

pH of 2% w/v aqueous solution at 25ºC 5.5-7.5 Protein content Min 5.00 % Carbohydrate content Min 70.00 % Loss on drying Max 5.00 % Appearance Cream to brownish yellow homogenous free flowing powder

Specifications

pH (1% w/v queous solution) 25°C 6.7 ñ 0.2 Typical cultural response after 18 - 48 hours at 35 °C E. Coli poor to good S.areus poor to good Indole test (tryptophan content) - ve

Specifications

Reaction to biuret Negative α – Amino nitrogen 5.5 ± 1.0 % Moisture Not more than 5.0% Sodium chloride less than 45 % Calcium 0,05 % Clarity (2% solution) Clear pH (2% solution) 5-7 Appearance Cream coloured amorphous free flowing powder

Specifications

pH (5% aqueous solution) 5.0 to 6.0 Total nitrogen % 13.8 - 15.8 Amino nitrogen % 4.7 - 6.7 Chloride (as NaCl) % Max 1.8 Sulfuric ashes % Max 7.0 Loss on drying % Max 6.0

Specifications

pH (1% w/v queous solution) 25°C 5.5 .721 Typical cultural response after 18 - 48 hours at 35 °C E. Coli good Klebsiella good Proteus good S.areus good Biochemical test MR + ve VP ñ ve Nitrate reduction - ve Carbohydrate fermentation - ve

Specifications

Appearance Light brown colored powder Bile acid content Min 45% Sulfated ash 14-18% Loss on drying Max 5.0% pH (2% solution in water) 6.0-8.0

Specifications

EEO 0.05 - 0.13 Gelling temperature (1.5%) 34.5 - 37.5°C Sulfate (SO4) Max 0.3 % Ash content Max 1 % Moisture content Max 10 % Appearance White crystalline powder